Effect of treatment on CL development was analyzed using the Chi Square procedure of SAS. Blood samples were collected via, jugular venipuncture, from day 0 until day 14 for analysis of serum progesterone and leptin during the infusion period. Gross morphology of CL tissue was recorded and either snap frozen in liquid nitrogen or paraffin embedded for microscopic evaluation. After 14 days, the osmotic pump and the remaining ovary were removed for analysis. Pumps containing treatments, C, AL, or IgG, were placed in apposition to the ovarian-uterine vascular plexus for a 14-day infusion of treatment at a flow rate of 0.5 µl/hr. On day 10 of the 3rd estrous cycle does were synchronized (PGF 2 α administration) for the surgical insertion of an osmotic, infusion pump at estrus (day 0). Females were checked twice daily for classical, behavioral estrus, using a cryptorchid male, for 2 consecutive cycles. All does were unilaterally ovariectomized to control for site of ovulation. Nine cycling crossbred does of similar age were randomly allocated to one of three treatment groups: Control, rabbit Anti-Leptin antibody, and rabbit IgG antibody. Therefore, it is hypothesized that blocking the action of ovarian leptin will disrupt luteal development in the caprine ovary. Collectively, the evidence suggests that leptin may be involved in CL development. Furthermore, it was determined that leptin influenced angiogenesis in the developing CL by upregulating the expression of angiogenic stimulators, i.e., VEGF and FGF. ![]() ![]() Previously, we reported the expression of both leptin and its receptor in the developing caprine CL, a period of time during which angiogenic activity is highest. Leptin is an important metabolic hormone known to have both reproductive and angiogenic properties. Common luteal defects include abnormal luteal development and decreased progesterone production, which are attributed in part by abnormal luteal angiogenesis. Corpora luteal (CL) defects contribute to reproductive failure issues and are believed to account for approximately 65% of miscarriages.
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